β-xylosidase Assay Kit (spectrophotometric/24 samples)

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Home > Biochemical reagent kit > Biochemical Kit > Sugar metabolism

β-xylosidase Assay Kit (spectrophotometric/24 samples)

β-xylosidase Assay Kit (spectrophotometric/24 samples)

Description

Description

Name:β-xylosidase Assay Kit

Measuring method: Spectrophotometric method

Specification: 50 tubes/24 samples

Storage conditions: low temperature and light protection

Validity: 6 months

Note: Choose 2-3 samples with large expected differences for pre-calibration before formal determination.

Measurement significance:

β-xylosidase (EC 3.2.1.37) is present in organisms such as plants, bacteria and fungi, and is a key enzyme catalysing the degradation of xylan-like hemicelluloses, and the product xylose can be used as a carbon source in microbial fermentation. In addition, β-xylosidase can be used as a biological bleaching agent applied in the paper industry, which is environmentally friendly than the traditional bleaching method and has a wide range of applications.

Measurement principle:

β-xylosidase catalyses the production of p-nitrophenol by p-nitrophenol-β-D-xyloside, p-nitrophenol has a characteristic absorption peak at 405nm, and the rate of increase of light absorption at 405nm can be measured to calculate the activity of β-xylosidase.

Provide your own lab supplies and apparatus:

Balance, low temperature centrifuge, visible spectrophotometer, 1 mL glass cuvette and distilled water.

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