Name: Urease(UE) Assay Kit
Method:Micro assay
Specification: 100 tubes/48 samples
Storage condition: low temperature and keep away from light
Validity: 6 months
Note: 2-3 samples with large expected differences must be taken for pre-calibration before formal measurement.
Significance:
UE can hydrolyse urea to produce ammonia and carbonic acid.UE activity is positively correlated with organic matter content, total nitrogen and quick nitrogen content, reflecting the nitrogen status.
Principle of Determination:
Determination of NH3-N produced by urease hydrolysis of urea using the indophenol blue colourimetric method.
Instruments and supplies to be provided on your own:
Visible spectrophotometer/enzymometer, water bath, adjustable pipettes, micro quartz cuvettes/96-well plates, mortar and pestle, ice, toluene (no courier allowed) and distilled water.
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