Reducing Sugar (RS)Content Assay Kit (Micromethod /48 samples)

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Home > Biochemical reagent kit > Biochemical Kit > Sugar metabolism

Reducing Sugar (RS)Content Assay Kit (Micromethod /48 samples)

Reducing Sugar (RS)Content Assay Kit (Micromethod /48 samples)

Description

Description

Name:Reducing Sugar (RS)Content Assay Kit

Determination Method：Micro-method

Specification: 100 tubes/48 samples

Storage conditions: low temperature and light protection

Valid period: 6 months

Note: Before the official determination, 2-3 samples with large expected differences must be taken for pre-calibration.

Significance:

Reducing sugars are widely found in animals, plants, microorganisms and cultured cells. Reducing sugars in plants mainly include glucose, fructose and maltose, which are the most common monosaccharides and disaccharides. Glucose and fructose are not only the main substrates for respiration, but also the substrates for further synthesis of sucrose, starch and cellulose.

Principle of Determination:

Heating promotes the formation of brown-red amino compounds from 3,5-dinitrosalicylic acid and reducing sugar in alkaline solution, with characteristic absorption peak at 540nm; within a certain concentration range, the amount of reducing sugar in the sample can be found out according to the standard curve, as the content and absorbance at 540nm are in linear relationship.

Instruments and supplies to be provided:

Visible spectrophotometer/enzymometer, water bath, adjustable pipette, micro-quartz cuvette/96-well plate, mortar, distilled water.

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