Nitrate Reductase（NR）Assay Kit （Spectrophotometry /48 samples）

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Home > Biochemical reagent kit > Biochemical Kit > Nitrogen metabolism

Nitrate Reductase（NR）Assay Kit （Spectrophotometry /48 samples）

Nitrate Reductase（NR）Assay Kit （Spectrophotometry /48 samples）

Description

Description

Name：Nitrate Reductase（NR）Assay Kit

Measurement method: Spectrophotometric method

Specification: 50 tubes/48 samples

Storage condition: low temperature and keep away from light

Validity: 6 months

Note: Select 2-3 samples with large expected differences for pre-calibration before formal measurement.

Measurement significance:

NR (EC 1.7.1.3) is widely found in plants and is a key enzyme in the conversion of plant nitrate nitrogen to ammoniacal nitrogen, as well as an inducible enzyme, which has an impact on crop yield and quality.

Principle of Determination:

NR catalyses the reduction of nitrate to nitrite, NO3ˉ +NADH+H+→ NO2ˉ +NAD+ +H 2 O; the generated nitrite can be quantitatively generated with p-aminobenzenesulphonic acid and α-naphthylamine to produce red azo compounds in acidic conditions; the generated red azo compounds have the maximum absorption peak at 540 nm, which can be measured by spectrophotometric method.

Apparatus and supplies to be provided:

Visible spectrophotometer, water bath, benchtop centrifuge, 1 mL glass cuvette, mortar and pestle, ice and distilled water.

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