Name: Micro Free Aliphatic Acid Content Assay Kit
Determination method: Spectrophotometric method
Specification: 50 tubes/24 samples
Storage conditions: low temperature and light protection
Validity: 6 months
Note: Choose 2-3 samples with large expected differences for pre-calibration before formal determination.
Measurement significance:
FFA is both a product of fat hydrolysis and a substrate for fat synthesis.The concentration of FFA is related to lipid metabolism, glucose metabolism, endocrine function, and can also reflect the quality changes in food storage.
Measurement principle:
Under weak acidic conditions, FFA reacts with copper salt to produce copper soap, with a characteristic absorption peak at 715nm, within a certain range of free fatty acid content and the degree of colour development is linear.
Self-contained apparatus and supplies:
Mortar and pestle, benchtop centrifuge, shaker, visible spectrophotometer, 1 mL glass cuvette.
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