Name:Hemicellulase Content Assay Kit
Determination method: Micro method
Specification: 100 tubes/96 samples
Storage conditions: low temperature and light protection
Validity period: 6 months
Note: Choose 2-3 samples with large expected differences for pre-calibration before formal determination.
Measurement significance:
Hemicellulose is a mixture of polysaccharides tightly bound to cellulose in the plant cell wall, which is the main component constituting the primary cell wall, widely found in plants, and is a new type of available energy.
Principle of Determination:
Hemicellulose is converted into reducing sugar after acid treatment, and DNS generates reddish brown substance, with characteristic absorption peak at 540nm, and the absorbance value reflects the content of hemicellulose.
Instruments and supplies to be provided on your own:
Balance, 40 mesh sieve, constant temperature water bath, oven, visible spectrophotometer/enzymometer, micro-quartz cuvette/96-well plate
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