Glucose-6-Phosphatase Assay Kit (Spectrophotometry /48 samples)

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Home > Biochemical reagent kit > Biochemical Kit > Gluconeogenesis

Glucose-6-Phosphatase Assay Kit (Spectrophotometry /48 samples)

Glucose-6-Phosphatase Assay Kit (Spectrophotometry /48 samples)

Description

Description

Name: Glucose-6-Phosphatase Assay Kit

Measurement method: Spectrophotometric method

Specification: 50 tubes/48 samples

Storage condition: low temperature and keep away from light

Validity: 6 months

Note: Select 2-3 samples with large expected differences for pre-calibration before formal measurement.

Measurement significance:

Glucose-6-phosphatase ((glucose 6 phosphatase, G6Pase, EC 3.1.3.9) is widely found in animals, plants, microorganisms and cells, it is the limiting enzyme for the hydrolysis of glucose-6-phosphate to produce glucose in the process of gluconeogenesis, and it plays an important role in ensuring the dynamic balance of blood glucose.

Principle of Measurement:

G6P catalyses the generation of glucose from glucose-6-phosphate, and then the enzyme and glucose dehydrogenase catalyse the reduction of NAD+ to NADH. The rate of NADH generation is measured at 340 nm, which reflects the activity of G6P.

Instruments and supplies to be provided:

UV spectrophotometer, benchtop centrifuge, adjustable pipette, 1 mL quartz cuvette, mortar and pestle, ice and distilled water.

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