Name: Free Cholestenone(FC) Content Assay Kit
Determination method: Spectrophotometric method
Specification: 50 tubes/48 samples
Storage conditions: low temperature and light protection
Validity: 6 months
Note: Choose 2-3 samples with large expected differences for pre-calibration before formal determination.
Measurement significance:
FC is the main component of cell membrane, and is also an important raw material for the synthesis of adrenocorticotropic hormone, sex hormone, bile acid and vitamin D. FC concentration can be used as an indicator of lipid metabolism.
Principle of measurement:
FC oxidase catalyses FC to generate △4-cholestenone and H2O2, peroxidase catalyses H2O2, 4-aminoantipyrine and phenol to generate red quinone compounds, with absorption peaks at 500nm, and its colour depth is proportional to the FC content.
Bring your own instruments and supplies:
Visible spectrophotometer, water bath, adjustable pipette gun, 1 mL glass cuvette, isopropyl alcohol and distilled water.