| Name: |
Chicken HSP70(Heat Shock Protein 70 high senstivity) ELISA Kit |
| other name: |
DAAP-21F2.7 ELISA Kit, FLJ54328 ELISA Kit, HSP70-1B ELISA Kit, HSP70-2 ELISA Kit, HSPA1A ELISA Kit, heat shock 70kD protein 1B ELISA Kit |
| Adaptation Species: |
Chicken |
| Specification: |
48T/96T |
| Storage: |
Storage at 2-8℃ for 6 months |
| UniProt ID: |
P08106 |
| Appilcation: |
The Chicken Heat Shock Protein 70 (HSP-70) enzyme-linked immunosorbent assay (elisa) kit allows for the in vitro quantitative determination of Chicken Heat Shock Protein 70 (HSP-70) concentration in plasma, tissue homogenates and other biological fluids. |
| method: |
double-anti-centering method |
| Detection range: |
0.156-10ng/ml |
| Sensitivity: |
< 0.094ng/ml |
| standard curve: |
 |
| recovery rate: |
A certain amount of chicken heat shock protein 70 (HSP-70) was admixed into the samples listed in the table below and the recoveries were calculated by comparing the measured values with the expected amount of chicken heat shock protein 70 (HSP-70) in the samples.
| |
Scope of Recycling(%) |
average value(%) |
| plasma(n=5) |
86-104 |
97 |
| EDTA plasma(n=5) |
89-101 |
95 |
| heparin plasma(n=5) |
85-105 |
94 |
|
| linearity: |
The linearity of the kit was determined by assaying samples mixed with appropriate concentrations of chicken heat shock protein 70 (HSP-70) and their serial dilutions, and the linearity was expressed as a percentage of the assayed concentration relative to the expected.
| Sample |
1:2 |
1:4 |
1:8 |
| plasma(n=5) |
87-102% |
92-104% |
87-105% |
| EDTA plasma(n=5) |
83-94% |
86-101% |
84-100% |
| heparin plasma(n=5) |
85-98% |
80-98% |
86-99% |
|
| coefficient of variation(%): |
Intra-lot variance: CV<8% Inter-lot variance: CV<10% |
| caveat: |
For scientific use only |