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Name: Amino Acid (AA) Content Test Kit Determination method: Micromethod Measuring specifications: 100T/96S Storage conditions: Store at low temperature and away from light Validity period: 6 months Note: Before formal measurement, select 2-3 samples with large expected differences for pre-determination. Measuring meaning: Animal livers and kidneys are the main organs for amino acid metabolism, so changes in amino acids in urine can best reflect the physiological status of the liver and kidneys. In addition, amino acids can also respond to burns, typhoid fever and other conditions. The amino acid content in plants is of great significance for studying changes in nitrogen metabolism of plants under different conditions and at different growth and development stages, as well as plant absorption, transportation, assimilation and nutritional status of nitrogen. Determination principle: The α-amino group of amino acids can react with ninhydrin to produce a blue-violet compound with a characteristic absorption peak at 570 nm; the amino acid content can be calculated by measuring the absorbance at 570 nm. Bring your own instruments and supplies: Desktop centrifuge, water bath, UV spectrophotometer/plate reader, micro quartz cuvette/96-well plate, adjustable pipette, mortar, absolute ethanol, ice and distilled water. |
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