Acetyl Co-A Assay Kit (Spectrophotometry /48 samples)

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Home > Biochemical reagent kit > Biochemical Kit > Tricarboxylic acid cycle

Acetyl Co-A Assay Kit (Spectrophotometry /48 samples)

Acetyl Co-A Assay Kit (Spectrophotometry /48 samples)

Description

Description

Name: Acetyl Co-A Assay Kit

Determination method: Spectrophotometric method

Specification: 50 tubes/48 samples

Storage condition: low temperature and keep away from light

Validity: 6 months

Note: Select 2-3 samples with large expected differences for pre-calibration before formal measurement.

Measurement significance:

Acetyl Coenzyme A is widely found in animals, plants, microorganisms and cultured cells. It is an important intermediate metabolite produced during the metabolism of energy substances in organisms. It is a pivotal substance in the metabolism of energy substances in the body. The three major nutrients, sugar, fat and protein, converge into a common metabolic pathway - the tricarboxylic acid cycle and oxidative phosphorylation - through acetyl coenzyme A. Through this pathway, carbon dioxide and water are generated by complete oxidation, releasing energy for ATP synthesis. In addition, acetyl coenzyme A is a precursor for the synthesis of fatty acids, ketone bodies, cholesterol and its derivatives, and other physiologically active substances.

Principle of Assay:

Malate dehydrogenase catalyses the formation of oxaloacetate and NADH from malate and NAD, while citrate synthase catalyzes the formation of citrate and coenzyme A from acetyl coenzyme A and oxaloacetate. Using the coupling reaction of malate dehydrogenase and citrate synthase, the amount of acetyl coenzyme A is proportional to the rate of formation of NADH, and the rate of increase in absorbance at 340 nm is a reflection of the level of acetyl coenzyme A content.

Instruments and supplies to be provided:

UV spectrophotometer, water bath, benchtop centrifuge, adjustable pipette, 1 mL quartz cuvette, mortar and pestle, ice and distilled water.

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