Name: Soil Alkaline Phosphatase (S-AKP/ALP) Assay Kit
Determination method: Micro method
Specification: 100T/96S
Storage condition: low temperature and light protection
Validity period: 6 months
Note: Select 2-3 samples with large expected differences for pre-calibration before formal measurement.
Measurement significance:
Soil phosphatase is a class of enzymes that catalyses the mineralization of soil organic phosphorus compounds, and its activity directly affects the decomposition and transformation of organic phosphorus and its biological effectiveness in soil, and is an indicator for evaluating the direction and intensity of soil phosphorus biotransformation. Soil phosphatase is significantly affected by soil carbon, nitrogen content, effective phosphorus content and pH. Usually, according to its optimum pH range, it is divided into three types of phosphatases: alkaline, neutral and acidic.
Principle of Determination:
In an alkaline environment, S-AKP/ALP catalyses the hydrolysis of disodium benzene phosphate to produce phenol and disodium hydrogen phosphate, and S-AKP/ALP activity can be calculated by measuring the amount of phenol produced.
Self-contained instrumentation and supplies:
Visible photometer/enzymometer, micro glass cuvettes/96-well plates, benchtop centrifuge, 37°C incubator, analytical balance, adjustable pipettes, ice, distilled water, anhydrous ethanol and toluene.
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