Soil Alkaline Phosphatase （S-AKP/ALP） Assay Kit (spectrophotometric/48 samples)

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Home > Biochemical reagent kit > Biochemical Kit > Soil

Soil Alkaline Phosphatase （S-AKP/ALP） Assay Kit (spectrophotometric/48 samples)

Soil Alkaline Phosphatase （S-AKP/ALP） Assay Kit (spectrophotometric/48 samples)

Description

Description

Name: Soil Alkaline Phosphatase （S-AKP/ALP） Assay Kit

Determination method: Spectrophotometric method

Specification: 50 tubes/48 samples

Storage conditions: low temperature and light protection

Validity: 6 months

Note: Select 2-3 samples with large expected differences for pre-calibration before formal measurement.

Measurement significance:

Soil phosphatase is a class of enzymes that catalyses the mineralization of soil organic phosphorus compounds, and the level of its activity directly affects the decomposition and transformation of organic phosphorus and its bioeffectiveness in soil, and it is an indicator for evaluating the direction and intensity of soil phosphorus biotransformation. Soil phosphatase is significantly affected by soil carbon, nitrogen content, effective phosphorus content and pH. Usually, according to its optimum pH range, it is divided into three types of phosphatases: alkaline, neutral and acidic.

Principle of Determination:

In an alkaline environment, S-AKP/ALP catalyses the hydrolysis of disodium benzene phosphate to produce phenol and disodium hydrogen phosphate, and S-AKP/ALP activity can be calculated by measuring the amount of phenol produced.

Self-contained instruments and supplies:

Visible spectrophotometer, benchtop centrifuge, 37°C constant temperature incubator, analytical balance, adjustable pipette, 1 mL glass cuvette, ice, distilled water, anhydrous ethanol and toluene.

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